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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has been noticed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial part in promoting mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been described. Two further splice variants were being documented but in depth nucleotide sequence info is just not yet offered.

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Blocking peptides are peptides that bind precisely towards the goal antibody and block antibody binding. These peptide typically consists of the epitope acknowledged with the antibody.

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This information outlines even further feasible optimization of cellular and immunoassay parameters to guarantee the best possible effects are...

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This manual outlines more probable optimization of mobile and immunoassay parameters to be certain the best possible final results are...

Antibodies sure to the blocking peptide now not bind on the epitope on the concentrate on protein. By evaluating the staining from the blocked antibody compared to the antibody by itself, one can see which staining is distinct.

Focus on Details Cyclin E belongs to the extremely conserved cyclin loved ones, whose members are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins functionality check here as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation patterns which lead to your temporal coordination of each and every mitotic party. Cyclin E types a complex with and functions for a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S stage boundary and is also degraded as cells development by S phase.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...

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